With a sweeping panorama of the prairie all around as you drive US 441 across Paynes Prairie, one spot lets you get out and see it up close: the Ecopassage Observation Boardwalk.
From Interstate 75, take the Micanopy exit and drive east to US 441. Turn left and watch for the park entrance on your right within a mile. Pass that entrance and continue 3.5 miles north along US 441, passing the Bolens Bluff trailhead. Stay in the right lane and slow down as the highway descends into the prairie so you can use the designated pulloff to park at the beginning of the boardwalk.